| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kung, Alexis D (11)
|
MRBGNW |
33.59 |
32.17 |
-1.42 |
| 1 |
Foote, Brianna R (11)
|
MRAGMV |
32.88 |
32.93 |
0.05 |
| 2 |
Hendrickson, Riley D (11)
|
MRYMID |
34.11 |
32.98 |
-1.13 |
| 3 |
Aikman, Saskia M (11)
|
MRYMID |
34.80 |
33.01 |
-1.79 |
| 4 |
Scanga, Adriana C (11)
|
MRCOND |
33.45 |
33.32 |
-0.13 |
| 5 |
Wachs, Lauren B (11)
|
MREAST |
33.63 |
33.68 |
0.05 |
| 6 |
McGourty, Molly F (11)
|
MRBGNW |
33.58 |
33.69 |
0.11 |
| 7 |
LePage, Charlotte G (11)
|
MREAST |
35.49 |
34.11 |
-1.38 |
| 8 |
DeSiena, Kaitlyn (11)
|
MRCFJV |
35.28 |
34.13 |
-1.15 |
| 9 |
DeGeorge, Sadie (11)
|
MRNYSA |
34.59 |
34.41 |
-0.18 |
| 10 |
Santos, Isabelle G (11)
|
MRPAC |
35.80 |
35.09 |
-0.71 |
| 11 |
Suzuki, Riko (11)
|
MRYMID |
35.60 |
35.16 |
-0.44 |
| 12 |
Chen, Ella Y (11)
|
MREAST |
35.99 |
35.63 |
-0.36 |
| 13 |
Calone, Eliana R (11)
|
MRMWDA |
36.07 |
35.66 |
-0.41 |
| 14 |
Cimorelli, Kathryn A (11)
|
MRNDAC |
34.47 |
36.02 |
1.55 |